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KMID : 1134820220510111171
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Journal of the Korean Society of Food Science and Nutrition
2022 Volume.51 No. 11 p.1171 ~ p.1177
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Development and Validation of an Analytical Method for the Detection of Resveratrol and trans-Scirpusin A as Functional Ingredients Smilax sieboldii Extract
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Kwon Jin-Gwan
Jung Yeon-Woo
Choi Yun-Hyeok
Lee Ji-Eun
Jeong Won-Sik
Lee Jung-A
Choi Chun-Whan
Ahn Eun-Kyung
Choi Yong-Mun
Hong Seong-Su
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Abstract
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This study was undertaken to establish an analytical method using high-performance liquid chromatography (HPLC). HPLC for the standard determination of resveratrol and trans-scirpusin A as functional ingredients in Smilax sieboldii extract. We evaluated the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) of various analytical methods for detecting resveratrol and trans-scirpusin A using HPLC. The specificity was confirmed by the chromatogram obtained using the HPLC analytical method. Also, the results of UV and the coefficient of correlation (R2) obtained was 0.999, which confirmed that this was a suitable analysis with high linearity. The LOD was 0.98, 0.49 μg/mL, and LOQ was 2.98, 1.48 μg/mL, which was confirmed as a suitable limit level for the analysis of resveratrol and trans-scirpusin A content in the S. sieboldii extract. The recovery of resveratrol and trans-scirpusin A content was determined to be 98.77±0.73∼99.24±1.47% and 98.45±1.18∼99.45±1.66%, respectively, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.67∼0.87%, 1.18∼1.33% and 0.93∼1.22%, 1.33∼2.27%, respectively, for trans-scirpusin A, for the relative standard deviation. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of resveratrol and trans-scirpusin A in S. sieboldii extract.
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KEYWORD
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Smilax sieboldii, resveratrol, trans-scirpusin A, HPLC, analytical method validation
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